Chemical Kinetics by ¹³C DNP NMR
Steven Reynolds, Devendra Babu Nama and Damir Blazina
Oxford Instruments Molecular Biotools. Ltd, Oxon, UK
The huge enhancement of the signal to noise ratio (SNR) afforded by DNP NMR allows direct detection of several low abundance nuclei (for example, 13C or 15N) in a single scan. By using small flip angles the enhanced magnetisation can be monitored over time to follow kinetic processes. An example is shown here for a chemical reaction.
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Deoxynucleotide Triphosphates and Oligonucleotides by ³¹P DNP NMR
Debadeep Bhattacharyya, Devendra Babu Nama and Steven Reynolds
Oxford Instruments Molecular Biotools. Ltd, Oxon, UK
The highly enhanced signal to noise ratio obtained by DNP NMR makes it possible to directly detect certain nuclei (for example, 31P) in only one scan and also in very low concentrations. Such methods allow us to obtain important structural information of bio-molecules at very low concentrations. In this application note, we report observation of 31P nuclei in microgram amounts of deoxynucleotide triphosphates. In addition the 31P NMR spectrum was observed for less than 200nmol of 12-mer oligonucleotides.
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Characterising Solid-State DNP
Rob Slade, Graham Hutton and Damir Blazina
Oxford Instruments Molecular Biotools. Ltd, Oxon, UK
DNP NMR yields greatly enhanced resonances in solution-state NMR spectra, overcoming the poor sensitivity of this very versatile analytical technique. To maximise the benefits that this technique can offer, it is necessary to understand and optimise the solid-state polarisation. We present a number of investigations into this process and discuss the implications for post-dissolution NMR measurements.
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Influence of Trityl Radical on the DNP Process
Damir Blazina, Steven Reynolds and Rob Slade
Oxford Instruments Molecular Biotools Ltd., Tubney Woods, Oxon, UK
A key component in Dynamic Nuclear Polarisation is the trityl radical, which acts as the source of unpaired electrons. The polarisation process depends on the interactions between the radical and the molecules of interest, making it important to choose an appropriate radical for each sample.
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DNP Dissolutions with Methanol
Damir Blazina, Vladimir Datskov, Steven Reynolds and Rob Slade
Oxford Instruments Molecular Biotools. Ltd, Oxon, UK
DNP NMR yields greatly enhanced resonances in solution-state NMR spectra, overcoming the poor sensitivity of this very versatile analytical technique. This makes it possible to directly detect low abundance nuclei such as 13C and 15N in only one scan and use 13C NMR in the currently unusual situation of detecting minor components in mixtures.
Using methanol as the dissolution solvent in DNP NMR enables the user to bring the benefits of this approach to some hydrophobic samples, or those that are incompatible with water. In addition, the flow properties of methanol lead to faster sample transfer, improved spectral line shape and better signal-to-noise.
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