HyperSense® - the in-vitro DNP Polariser

Oxford Instruments is currently focusing on commercialisation activities and will continue to sell, promote and support the HyperSense instrument.

HyperSense allows the user to drive NMR sensitivity to levels never seen before. The sensitivity improvements attainable with 13C, 15N, 29Si and 31P have the potential to increase the breadth of Nuclear Magnetic Resonance (NMR) experiments and your applications.

Examples are:

Shorter data collection times and/or the use of much smaller amounts of sample material
Benefits of quaternary carbons that will improve structure confirmation and elucidation. This could provide more reliable NMR spectral assignments in a shorter time
The ability using direct detection of ¹³C to identify complex biomixtures in metabonomics
Using the enhanced signal to carry out chemical kinetic experiments
HyperSense configures easily with your laboratory and existing spectrometer

Applications Library
Performance
Services
Specification
Publications

Applications Library

Click here to link to Applications Library - Downloadable applications notes, posters, articles and more ...

Nuclei available:	¹³C, ¹⁵N, ²⁹Si, ³¹P and other spin 1/2 nuclei
Sample polarisation times:	Typically 15 minutes to 6 hours
NMR spectrometer compatibility:	All current spectrometer platforms
NMR RF probe requirements:	Operation with 5 and 10mm probes
Dissolution solvents:	Water and methanol
Polarising agent:	‘Trityl radical’, typical usage 0.2-5mg per sample

Compressed air/nitrogen:	6 bar min
Helium gas:	6 bar min, grade 99.999% pure from cylinder
Exhaust line:	Vacuum exhaust line or suitable safety compliant filter
Electrical:	Europe: 230V, 50Hz, 2.5A single phase and 415V, 50Hz 20A three phase USA: 110V, 60Hz, 3A single phase and 208V, 60Hz, 20A three phase Japan: 100V, 50-60Hz, 3A single phase and 200V, 50-60Hz, 20A three phase

Microwave source:	Integrated 94GHz with 0.5GHz sweep (user selectable) up to 100mW
Magnetic field strength:	3.35T actively shielded
Sample temperature:	Selectable < 3.9K, ≥1.4K¹
Helium refill volume:	65L from minimum level
Nitrogen refill volume:	60L from minimum level
Helium consumption during dissolutions:	Typical <2L per dissolution²
Minimun operating ceiling height:	<3.0m
System weight including cryogens:	~ 600kg
Trigger output for NMR spectrometer:	5V signal

Dimensions

Footprint:	W 1668mm (~ 66") x D 1038mm (~ 41")
Height:	2860mm (~ 113")

(1) As measured by instrument control system with 65% helium insert level at microwave power 100mW over 30 minutes

(2) Averaged over 10 repeatable runs with a temperature of 1.4K over a 6 hour polarisation and dissolution, 100mW microwave power with helium level at 65%

Author	Journal	Reference	Title
Bowen S.; Zeng H.; Hilty C.	Anal. Chem.	2008, 80, 5794-5798	Chemical Shift Correlations from Hyperpolarized NMR by Off-Resonance Decoupling
Bowen S.;Hilty C.	Angew. Chem. Int. Ed.	2008, 47, 5235-5237	Time-Resolved Dynamic Nuclear Polarization Enhanced NMR Spectroscopy
Reynolds S.; Patel H.	Applied Magnetic Resonance	2008, 34, 495-508	Monitoring the Solid-State Polarization of 13C, 15N, 2H, 29Si and 31P
Day I. J.; Mitchell J. C.; Snowden M. J.; Davis A. L.	Applied Magnetic Resonance	2008, 34, 453-460	Investigation of the Potential of the Dissolution Dynamic Nuclear Polarization Method for General Sensitivity Enhancement in Small-Molecule NMR Spectroscopy
Emwas A.-H.; Saunders M.; Ludwig C.; Günther U. L.	Applied Magnetic Resonance	2008, 34, 483-494	Determinants for Optimal Enhancement in Ex Situ DNP Experiments
Mishkovsky M.; Frydman L.	ChemPhysChem	2008, 9, 2340-2348	Progress in Hyperpolarized Ultrafast 2D NMR Spectroscopy
Gabellieri C.; Reynolds S.; Lavie A.; Payne G. S.; Leach M. O.; Eykyn T. R.	J. Am. Chem. Soc.	2008,130, 4598-4599	Therapeutic Target Metabolism Observed Using Hyperpolarized 15N Choline
Saunders M. G.; Ludwig C.;Günther U. L.	J. Am. Chem. Soc.	2008, 130, 6914-6915	Optimising the Signal Enhancements In Cryogenic ex situ DNP-NMR Spectroscopy
Merritt M. E.; Harrison C.; Kovacs Z.; Kshirsagar P.; Malloy C. R.; Sherry A. D.	J. Am. Chem. Soc.	2007, 129, 12942-12943	Hyperpolarized 89Y Offers the Potential of Direct Imaging of Metal Ions in Biological Systems by Magnetic Resonance
Merritt M. E.; Harrison C.; Mander W.; Malloy C. R.; Sherry A. D.	Journal of Magnetic Resonance	2007, 189 (2), 280 - 285	Dipolar cross-relaxation modulates signal amplitudes in the 1H NMR spectrum of hyperpolarized [13C]formate
Day I. J.; Mitchell J. C.; Snowden M. J.; Davis A. L.	Journal of Magnetic Resonance	2007, 187(2), 216 - 224	Applications of DNP-NMR for the measurement of heteronuclear T1 relaxation times
Masafumi Harada; Hitoshi Kubo; Takamasa Abe; Hiroshi Maezawa; Hideki Otsuka	Jpn J Radiol	2010, 28, 173 - 179	Selection of endogenous 13C substrates for observation of intracellular metabolism using the dynamic nuclear polarization technique
Day I. J.; Mitchell J. C.; Snowden M. J.; Davis A. L.	Magn Reson. Chem.	2007, 45, 1018-1021	Co-acquisition of hyperpolarised 13C and 15N NMR spectra
Frydman L.; Blazina D.	Nature Physics	2007, 3, 415-419	Ultra fast two-dimensional nuclear magnetic resonance spectroscopy of hyperpolarized solutions
Merritt M. E.; Harrison C.; Storey C.; Jeffrey F. M.; Sherry A. D.; Malloy C. R.	PNAS	2007, 104, no. 50, 19773-19777	Hyperpolarized 13C allows a direct measure of flux through a single enzyme-catalyzed step by NMR